Journal: Environment international

Article Title: BaP/BPDE suppressed endothelial cell angiogenesis to induce miscarriage by promoting MARCHF1/GPX4-mediated ferroptosis.

doi: 10.1016/j.envint.2023.108237

Figure Lengend Snippet: Fig. 2. BPDE exposure suppressed HUVEC angiogenesis. HUVECs were exposed with 0, 0.5, 1.0, or 1.5 μM BPDE for 36 h. The levels of tube formation of HUVECs (A- B) and the protein levels of ANG-1 and VEGFA (C-D) were determined in HUVECs. The protein expression level of each protein band was normalized to its own loading control (ACTB) and plotted (D).

Article Snippet: The concentrations of VEGFA in serum samples were determined by ELISA using the Human VEGFA ELISA kits (RK00023, ABclonal, USA) according to the manufacturer’s instructions (Li et al. 2023).

Techniques: Expressing, Control

Journal: Environment international

Article Title: BaP/BPDE suppressed endothelial cell angiogenesis to induce miscarriage by promoting MARCHF1/GPX4-mediated ferroptosis.

doi: 10.1016/j.envint.2023.108237

Figure Lengend Snippet: Fig. 3. BPDE exposure induced HUVEC ferroptosis and then suppressed angiogenesis. (A-E) The levels of mRNAs of typical genes that function for iron importation (TFR and HFE), iron storage (FTH, FTL and FTMT), and iron exportation (FPN1 and CP) (A), intracellular free Fe2+ (B), and mRNAs and proteins of COX-2 and NOX-1 (C-D) were examined in 0, 0.5, 1.0, or 1.5 μM BPDE-exposed HUVECs. The protein expression level of each protein band was normalized to its own loading control (ACTB) and plotted (E-J) The levels of cell viability (CCK8) (E), cytotoxicity (LDH) (F), lipid peroxidation (MDA) (G), the total peroxidase activity (GPx activity) (H), intracellular ROS (I), and intracellular free Fe2+ (J) were examined in 0, 0.5, 1.0, or 1.5 μM BPDE-exposed HUVECs treated with 10 μM ferrostatin-1 (Fer-1, fer roptosis inhibitor). (K-O) Tube formation (K, L and N) and the protein levels of ANG-1 and VEGFA (M and O) were examined in 1.0 μM BPDE-exposed HUVECs treated with 10 μM Fer-1 or Erastin. All the value of each band density in experimental and control groups was normalized to that of its corresponding ACTB band.

Article Snippet: The concentrations of VEGFA in serum samples were determined by ELISA using the Human VEGFA ELISA kits (RK00023, ABclonal, USA) according to the manufacturer’s instructions (Li et al. 2023).

Techniques: Expressing, Control, Activity Assay

Journal: Environment international

Article Title: BaP/BPDE suppressed endothelial cell angiogenesis to induce miscarriage by promoting MARCHF1/GPX4-mediated ferroptosis.

doi: 10.1016/j.envint.2023.108237

Figure Lengend Snippet: Fig. 4. BPDE exposure reduced GPX4 expression level, promoted ferroptosis, and thus suppressed angiogenesis of HUVECs. (A) The protein level of GPX4 in BPDE- exposed HUVECs. (B-G) The levels of cell viability (CCK8) (B), the total peroxidase activity (GPx activity) (C), cytotoxicity (LDH) (D), lipid peroxidation (MDA) (E), intracellular free Fe2+ (F), and intracellular ROS (G) were examined in BPDE-exposed HUVECs with GPX4 overexpression. (H-J) Tube formation (H) and the protein levels of ANG-1 and VEGFA (I-J) were examined in GPX-overexpressed HUVECs with Erastin (10 μM) treatment. (K-N) Tube formation (K-L) and the protein levels of ANG-1 and VEGFA (M−N) were examined in BPDE-exposed HUVECs with overexpression or knockdown of GPX4. (O-P) Tube formation (O) and the protein levels of GPX4, VEGFR1, and VEGFR2 (P) in HUVECs treated with angiogenesis suppressor Sunitinib (10 nM) or Sorafenib (90 nM). All the value of each band density in experimental and control groups was normalized to that of its corresponding ACTB band.

Article Snippet: The concentrations of VEGFA in serum samples were determined by ELISA using the Human VEGFA ELISA kits (RK00023, ABclonal, USA) according to the manufacturer’s instructions (Li et al. 2023).

Techniques: Expressing, Activity Assay, Over Expression, Knockdown, Control

Journal: Environment international

Article Title: BaP/BPDE suppressed endothelial cell angiogenesis to induce miscarriage by promoting MARCHF1/GPX4-mediated ferroptosis.

doi: 10.1016/j.envint.2023.108237

Figure Lengend Snippet: Fig. 6. BPDE-DNA adduct, ferroptosis, and defective angiogenesis in decidual tissues were associated with RM. (A) The levels of BPDE-DNA adduct in HC and RM decidual tissues (each n = 30). (B-C) The protein levels of ANG-1 and VEGFA in HC and RM decidual tissues (each n = 12). (D) The levels of intracellular free Fe2+ in both HC and RM decidual tissues (each n = 30). (E-F) The mRNA and protein levels of COX-2 and NOX-1 in both HC and RM decidual tissues (each n = 12). (G) The protein levels of GPX4 and MARCHF1 in HC and RM villous tissues (each n = 12). Their protein band intensity was quantified and plotted. (H-I) IP assay analysis of the levels of GPX4-Ub and MARCHF1 that was pulled down by GPX4 in HC and RM tissues, with GPX4 and MARCHF1 protein levels shown in tissue lysate (Input) (each n = 12). (J-L) The correlation analysis of the protein levels of GPX4 with MARCHF1 (J), VEGFA (K), or ANG-1 (L) in HC (blue) and RM (red) groups (each n = 12). All the value of each band density in experimental and control groups was normalized to that of its corresponding ACTB band. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The concentrations of VEGFA in serum samples were determined by ELISA using the Human VEGFA ELISA kits (RK00023, ABclonal, USA) according to the manufacturer’s instructions (Li et al. 2023).

Techniques: Control

Journal: Environment international

Article Title: BaP/BPDE suppressed endothelial cell angiogenesis to induce miscarriage by promoting MARCHF1/GPX4-mediated ferroptosis.

doi: 10.1016/j.envint.2023.108237

Figure Lengend Snippet: Fig. 8. Supplement with murine Gpx4 could efficiently suppress ferroptosis, recover angiogenesis, and alleviate miscarriage in a BaP-exposed mouse model. (A) The scheme for construction of a BaP-exposed mouse model with miscarriage. (B) Embryo resorption (indicated by red arrows) in BaP-exposed mice with miscarriage (scale bar, 1 cm) and the average miscarriage rate (embryo resorption rates) in BaP-exposed mice (each n = 6). (C) The protein levels of murine Nox1, Cox2, Gpx4, Marchf1, Ang-1, and Vegfa in placental tissues of BaP-exposed mice (n = 6). (D) The scheme for construction of a miscarriage intervention model. (E) Embryo resorption (indicated by red arrows) in BaP-exposed mice with Gpx4 supplement (scale bar, 1 cm) and the average miscarriage rates (embryo resorption rates) in BaP- exposed mice with supplement with Gpx4 (each n = 6). (F) The protein levels of Nox1, Cox2, Gpx4, Marchf1, Ang-1, and Vegfa in BaP-exposed mice with supplement with Gpx4 (each n = 6). All the value of each band density in experimental and control groups was normalized to that of its corresponding ACTB band. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The concentrations of VEGFA in serum samples were determined by ELISA using the Human VEGFA ELISA kits (RK00023, ABclonal, USA) according to the manufacturer’s instructions (Li et al. 2023).

Techniques: Control

Journal: BMC pregnancy and childbirth

Article Title: Expression of insulin-like growth factor binding protein-3 in HELLP syndrome.

doi: 10.1186/s12884-023-06074-7

Figure Lengend Snippet: Fig. 1 Levels of IGFBP-3/IGF-1/TGF-β1/VEGF in maternal blood of three groups (*P < 0.05, **P < 0.01)

Article Snippet: The reagents comprised: Human IGFBP-3 ELISA kit (R&B, DY675, USA), IGF-1 ELISA kit (R&B, DY291-05, USA), TGF-β1 ELISA kit (R&B, DY240-5,USA), Human VEGF ELISA Kit (R&B, DY293B-5,USA), goat serum (Proteintech Inc., B900780, USA), Tween-20 (Sigma Inc., T2700, USA), substrate solution (R&B,DY999, USA), bovine serum albumin (Sigma, B2064-50,USA), Annexin V-FITC/PI apoptosis assay kit (Novizan, A21101, China), endothelial cell culture medium (ScienCell, 1001, USA), recombinant human IGFBP-3 protein (YiqiaoShenzhou Technology Co., Ltd., 10430-H07H, China).

Techniques:

Journal: BMC pregnancy and childbirth

Article Title: Expression of insulin-like growth factor binding protein-3 in HELLP syndrome.

doi: 10.1186/s12884-023-06074-7

Figure Lengend Snippet: Fig. 2 Levels of IGFBP-3/IGF-1/TGF-β1/VEGF in umbilical blood of three groups (*P < 0.05, **P < 0.01)

Article Snippet: The reagents comprised: Human IGFBP-3 ELISA kit (R&B, DY675, USA), IGF-1 ELISA kit (R&B, DY291-05, USA), TGF-β1 ELISA kit (R&B, DY240-5,USA), Human VEGF ELISA Kit (R&B, DY293B-5,USA), goat serum (Proteintech Inc., B900780, USA), Tween-20 (Sigma Inc., T2700, USA), substrate solution (R&B,DY999, USA), bovine serum albumin (Sigma, B2064-50,USA), Annexin V-FITC/PI apoptosis assay kit (Novizan, A21101, China), endothelial cell culture medium (ScienCell, 1001, USA), recombinant human IGFBP-3 protein (YiqiaoShenzhou Technology Co., Ltd., 10430-H07H, China).

Techniques:

Journal: BMC pregnancy and childbirth

Article Title: Expression of insulin-like growth factor binding protein-3 in HELLP syndrome.

doi: 10.1186/s12884-023-06074-7

Figure Lengend Snippet: Fig. 3 Correlation analysis between IGFBP-3 and IGF-1/TGF-β1/VEGF (**P < 0.01, Line 1: results of correlation analysis in maternal blood; Line 2: results of correlation analysis in umbilical blood. The correlation coefficient (r) and regression equation are shown in the figure

Article Snippet: The reagents comprised: Human IGFBP-3 ELISA kit (R&B, DY675, USA), IGF-1 ELISA kit (R&B, DY291-05, USA), TGF-β1 ELISA kit (R&B, DY240-5,USA), Human VEGF ELISA Kit (R&B, DY293B-5,USA), goat serum (Proteintech Inc., B900780, USA), Tween-20 (Sigma Inc., T2700, USA), substrate solution (R&B,DY999, USA), bovine serum albumin (Sigma, B2064-50,USA), Annexin V-FITC/PI apoptosis assay kit (Novizan, A21101, China), endothelial cell culture medium (ScienCell, 1001, USA), recombinant human IGFBP-3 protein (YiqiaoShenzhou Technology Co., Ltd., 10430-H07H, China).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Bovine Organospecific Microvascular Endothelial Cell Lines as New and Relevant In Vitro Models to Study Viral Infections

doi: 10.3390/ijms21155249

Figure Lengend Snippet: Evaluation of specific RNA marker expression by RT-qPCR in bovine endothelial cell lines and bovine aortic primary endothelial cells (BAEC).

Article Snippet: VEGFA production was measured using either the human VEGFA kit (R&D, ref DY293B), the murine VEGFA kit (R&D, ref DY493) or three different bovine VEGFA specific ELISA kits (Elisa Genie, ref BOEB0087; Cusabio, ref CSB-EL025833BO; Ray-Bio, ref ELB-VEGFA), according to the manufacturer’s instructions.

Techniques: Marker, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Bovine Organospecific Microvascular Endothelial Cell Lines as New and Relevant In Vitro Models to Study Viral Infections

doi: 10.3390/ijms21155249

Figure Lengend Snippet: Bovine genes and associated primers used for the cell lines characterization by RT-qPCR.

Article Snippet: VEGFA production was measured using either the human VEGFA kit (R&D, ref DY293B), the murine VEGFA kit (R&D, ref DY493) or three different bovine VEGFA specific ELISA kits (Elisa Genie, ref BOEB0087; Cusabio, ref CSB-EL025833BO; Ray-Bio, ref ELB-VEGFA), according to the manufacturer’s instructions.

Techniques: Sequencing